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Everything you need to know about our proteomics services

Frequently Asked Questions

Ordering & Pricing

How do I place an order?
Contact us at omixai@omixai.com with details about your project. We'll provide a customized quote and sample submission instructions.
What is the typical turnaround time?
Standard proteomics analysis takes 2-4 weeks from sample receipt. Rush services are available upon request.
What payment methods do you accept?
We accept bank transfers, purchase orders from academic institutions, and major credit cards for qualifying orders.

Sample Preparation

What sample types do you accept?
We accept cells, tissues, biofluids (plasma, serum, CSF), and protein lysates. Contact us for specific sample type requirements.
How much sample material do I need?
For tissue: 10-20mg, cells: 1-5 million, plasma/serum: 10-50μL. Lower amounts may be possible - please inquire.
How should I ship my samples?
Ship frozen samples on dry ice via overnight courier. We'll provide detailed shipping instructions and materials upon order confirmation.
What are the sample quality requirements?
Samples should be flash-frozen and stored at -80°C. Avoid freeze-thaw cycles. We can assess sample quality before proceeding with analysis.

Technical Details

What mass spectrometry platform do you use?
We use the Thermo Fisher Orbitrap Astral, providing ultra-high resolution (240,000 FWHM) and sensitivity for deep proteome coverage.
How many proteins can you identify?
We routinely identify 8,000-12,000 proteins in human cell lines and tissues with high confidence (FDR < 1%).
What quantification methods do you offer?
We support label-free quantification (LFQ), TMT/iTRAQ multiplexing, and SILAC approaches depending on your experimental design.
What bioinformatics analysis is included?
Standard analysis includes protein identification, quantification, statistical testing, GO enrichment, and pathway analysis. Advanced AI-powered analysis is available.

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Technical Documentation

Mass Spectrometry Platform

Thermo Fisher Orbitrap Astral

  • Resolution: 240,000 FWHM at m/z 200
  • Mass Accuracy: < 1 ppm
  • Scan Speed: 200 Hz
  • Dynamic Range: > 5 orders of magnitude
  • Sensitivity: Sub-femtomole detection limits

Standard Proteomics Workflow

1. Sample Preparation

Protein extraction, reduction, alkylation, and tryptic digestion using optimized protocols for each sample type.

2. Peptide Cleanup

C18 solid-phase extraction to remove salts and contaminants.

3. LC-MS/MS Analysis

90-120 minute gradient on nanoflow HPLC coupled to Orbitrap Astral with data-dependent acquisition (DDA).

4. Data Processing

Database searching with FragPipe or MaxQuant, FDR filtering at 1% protein and peptide level.

5. Quantification & Analysis

Label-free quantification (LFQ) or isobaric labeling (TMT), statistical analysis, pathway enrichment.

Typical Performance Metrics

Sample Type Proteins Identified Peptides Identified
Human cell line (1 μg) 8,000-10,000 80,000-100,000
Human tissue (10 mg) 9,000-12,000 90,000-120,000
Human plasma (10 μL) 500-800 5,000-8,000

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